Syllabus (MICROBIOLOGY)

Course Type: ME-3

Semester: 3

Course Code: BMCBMEB23T

Course Title: BACTERIOLOGY AND VIROLOGY (THEORY)

(L-P-Tu): 3-0-1

Credit: 4

Practical/Theory: Theory

Course Objective: To study the basics of microbiology with special emphasis on bacteriology. 2. To learn about bacteriophages and understand the classification, morphology, lifecycle of viruses along with their role in humans.

Learning Outcome: Upon successful completion of the course the candidate will be able to- 1. Gain knowledge about the bacterial taxonomy, molecular structure of bacteria, growth kinetics, different cell organelles and their detailed functions. 2. Acquire knowledge about the structure, multiplication and pathogenicity of different viruses.

MINOR COURSE-II

COURSE TITLE:

TOTAL HOURS: 45 CREDITS:3

Unit 1. Cell organization No. of hours: 14

Cell size, shape and arrangements, capsule, flagella and pili, Composition and detailed structure of gram- positive and gram- negative cell wall and archaeal cell wall, Structure, chemical composition and functions of bacterial and archaeal cell membranes, Ribosomes, inclusions, nucleoid, plasmids, structure, formation and stages of sporulation.

Unit 2. Bacterial growth No. of hours: 12

Culture media: Components of media, Synthetic or defined media, Complex media, enriched media, selective media, differential media, enrichment culture media

Pure culture isolation: Streaking, serial dilution and plating methods, cultivation, maintenance and stocking of pure cultures, cultivation of anaerobic bacteria.

Growth: Binary fission, phases of growth.

Unit 3. Introduction to Viruses No. of hours: 7

Properties of viruses; general nature and important features Subviral particles; viroids, prions and their importance Isolation and cultivation of viruses.

Unit 4. Structure and multiplication of viruses No. of hours: 12

Morphological characters: Capsid symmetry and different shapes of viruses with examples; Viral multiplication in the Cell: Lytic and lysogenic cycle.

Description of important viruses: salient features of the viruses infecting different hosts - Bacteriophages (T4), Plant (TMV), Human (HIV).

COURSE TITLE: BACTERIOLOGY AND VIROLOGY (PRACTICAL)

TOTAL HOURS: 30 CREDITS:1

1. Preparation of culture media for bacterial cultivation
2. Isolation of pure cultures of bacteria by streaking method
3. Enumeration of colony forming units (CFU) count by spread plate method/pour plate.
4. Determination of viral plaque assay.

SUGGESTED READINGS

1. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology. 9th edition. McGraw Hill Higher Education.
2. Dimmock, NJ, Easton, AL, Leppard, KN (2007). Introduction to Modern Virology. 6th edition, Blackwell Publishing Ltd.
3. Atlas RM. (1997). Principles of Microbiology. 2nd edition. WM. T. Brown Publishers
4. Madigan MT, Martinko JM, Dunlap PV and Clark DP (2014). Brock Biology of Micro-organisms. 14th edition. Pearson Education, Inc.
5. Carter J and Saunders V (2007). Virology; principles and Applications. John Wiley and Sons
6. Flint SJ, Enquist, LW, Krug, RM, Racaniello, VR Skalka, AM (2004) Principles of Virology, Molecular Biology, Pathogenesis and Control.2nd edition.ASM Press
7. Shors Teri (2013) Understanding Viruses 2nd edition Jones and Bartlett Learning Burlington USA
8. Pelczar Jr MJ, Chan ECS, and Krieg NR. (2004). Microbiology. 5th edition Tata McGraw Hill.
9. Tortora GJ, Funke BR, and Case CL. (2008). Microbiology: An Introduction. 9th edition Pearson Education.